The friendly talk show host addressed the baby issue during an interview on the Tonight Show With Jay Leno last fall, similarly explaining that kids just wouldn't be in the cards for the hot Hollywood couple.
"I don't want to have one. I don't want to have six. I don't want to have any," DeGeneres, 54, told Leno in the September interview. "They're precious to look at and I love them; we have nieces and I love them very much. [But I] don't want 'em."
DeGeneres also shot down rumors about wanting a family in her 2011 book, Seriously…I'm Kidding, explaining that the couple just didn't feel the urge to have little munchkins of their own.
"People are constantly asking Portia and me if we are going to have children," she wrote. "We thought about it. We love to be around children after they've been fed and bathed. But we ultimately decided that we don't want children of our own. There is far too much glass in our house."
DeGeneres and de Rossi, 40, tied the knot at their home in the summer of 2008, and have been happy to keep their home-life just the two of them.
In a recent interview with Out magazine, de Rossi admitted that though she has previously struggled with whether or not she would genuinely want children, her answer still hasn't changed.
"There comes some pressure in your mid-30s, and you think, Am I going to have kids so I don't miss out on something that other people really seem to love?" she mused in the Out interview. "Or is it that I really genuinely want to do this with my whole heart? I didn't feel that my response was 'yes' to the latter."
"You have to really want to have kids, and neither of us did," she continued. "So it's just going to be me and Ellen and no babies."